Overview

Jobin Yvon IBH decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrumental response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF. Tell me more.

DAS6, the latest version of our decay analysis software and a member of the Fluorescence Measurement and Analysis Studio, uses algorithms based on those proven over the years by users of our industry-leading v4.2 software. These algorithms have been repackaged using ActiveX technology to produce a modular 32-bit Windows™ application similar in structure to v4.2. The Foundation license comprises the main application workspace, and allows reconvolution analysis with up to four exponentials. Additional components include anisotropy, batch analysis, lifetime distribution, micellar quenching and energy transfer. For more information about these components see below.

Only Jobin Yvon IBH offers the following functionality in one easy to use package...

Functionality

Jobin Yvon IBH DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing the opportunity for fine-tuning of parameters where necessary. v4.2 users will note a dramatic increase in productivity due to the new mouse-driven interface and new .DAS file structure. The following packages and components can be licensed.

Foundation package (DAS-6-FSP)
The Foundation package contains reconvolution analysis with up to 4 exponentials, statistical and file manipulation utilities. All other DAS6 components require the Foundation package to operate.

Batch mode extension to foundation package (DAS-6-FSP-B)
Analyses datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring. This new module features full reconvolution with up to four exponentials and shift iteration. Parameters can be held common between datasets. All data and results are stored in a single data file and results are automatically tabulated for use in external spreadsheet applications.

Lifetime distribution (DAS-6-5)
Distribution of decay times and distribution plus additional exponential.

Fit to exponential series (DAS-6-7)
Exponential series analysis of up to 30 terms.

Anisotropy (DAS-6-8)
Impulse reconvolution of up to 2 correlation times and 4 fluorescence decay components. Each model parameter, including shift, can be fixed at a pre-determined value or optimised as part of the parameter fitting. The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.

Förster energy transfer (DAS-6-9)
2-D and 3-D Förster-type energy transfer with optional additional exponential component.

Yokota-Tanimoto energy transfer (DAS-6-10)
Yokoto-Tanimoto energy transfer with optional additional exponential component.

Micellar quenching (DAS-6-11)
Micellar quenching kinetics.

Global analysis (DAS-6-12)
Global analysis of up to 4 exponential components and 12 decay curves.


Frequently Asked Questions For v4.2 Users

Is DAS6 a proper Windows™ application?
Yes. DAS6 is a proper 32-bit application designed for the latest Microsoft operating systems, including Windows™ 98 and 2000. DAS6 is NOT based on Windows™ look-alike technology such as GEM.

Will my existing software protection device work with v6.1?
DAS6 does not require a software protection device ("dongle"). Like most other commercial software packages, DAS6 is distributed under a single-user license agreement, and requires a unique registration key to be entered during installation.

Can I still use the files created using my v4.2 software?
Yes. Although DAS6 introduces a new file structure, it also supports data files in v4.2 format. What's more, it is now much easier to import and export data in other file formats such as ASCII and Maestro.


System Requirements

The following PC specifications are required to run DAS6.

Pentium 133 or better, 32Mb RAM, 40Mb HD space, CD-ROM drive, SVGA (1024x768) graphics with minimum 256 colour support, mouse or other pointing device, Windows™ 95/98/98SE/Me/NT/XP (2000 not supported). Windows 98SE recommended.