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SPEX® FluoroLog® - Tau - Why do I need a Lifetime Spectrofluorometer?
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 Time
is Always on your side once your laboratory is equipped
with a Spex® Tau Lifetime Spectrofluorometer. Instantly,
Tau delivers the best of both worlds: steady-state and dynamic
measurements with just the twist of a knob. You get TRUE
picosecond performance for far less expenditure than any
other picosecond system.
Why
frequency domain?
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Measurements
are backed up by over a century of research and experience
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Uses a reference to monitor the modulation of the excitation
beam while also measuring the emission, so true differential
measurement is performed.
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Produces two observables - phase and modulation - for
determining the lifetime.
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Usually faster, easier, and requires less maintenance.
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Can be performed with a source that has a continuous output
compatible with steady-state measurements. Therefore,
a dual-purpose instrument can be manufactured with the
same light source for both.
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The differential measurements cancel out instrument artifacts
such as pulse-smearing through monochromators, that limit
the usefulness of time-domain measurements.
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Highest
sensitivity-even with a xenon source (cw laser optional)
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Flexibility with sources-no expensive pulse lasers are
needed
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Flattest frequency response over the full frequency range
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Fast selection of lifetime or steady-state operation
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Upgrades for any FluoroLog®-3 to lifetime capability
 In
the frequency domain, the excitation source has its intensity
modulated at a high frequency. As a result, the sample's
emission is also modulated, but out of phase with the excitation.
Both the phase-difference and the modulation of the emission
are directly linked to lifetime.
Tau
applications
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Lifetime
Heterogeneity
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Distributed Fluorescence Decays
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Anisotropy Decays
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Single and multi-component fluorescence decays
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Resolve picosecond lifetimes
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Deconvolve multiple spectra
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Watch the time-evolution of spectra
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Record time-resolved polarisation spectra
 Lifetime
Micro-Mapping System
Now, you can study
fluorescence dynamics, which are independent of fluorophore
concentration, and gain more information about molecular
motion, sizes, local environment, interaction, and binding,
with the ability to discriminate microscopic locations in
your sample. With the power of confocal microscopy, you
can get sharp images and determine localised interactions
in cells and dynamics of cellular structures. Steady-state
spectra of microscopic areas on your sample are a snap,
too!
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