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TCSPC Confocal Lifetime Microscopy for Bio- and Nanotechnology
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 Time-resolved
microscopy is the ultimate tool for investigating dynamic events
in cells and subcellular structures. Its applications previously
have been limited at wavelengths longer than 370 nm because microscope
optics do not transmit in the deep UV. HORIBA Jobin Yvon, the leader
in fluorescence spectroscopy, introduces a filter-based confocal
system (the DynaMic™) to measure fluorescence lifetime and intensity
directly under the microscope in the deep UV and visible (240–850
nm). With TCSPC’s unequalled sensitivity, this microscope-fluorescence
system finds wide applications in biotechnology and nanotechnology.
The DynaMic™ is especially useful for intrinsic protein fluorescence
measurements, such as distinguishing tryptophan and tyrosine, with
the 295 nm LED.
Uses for the TCSPC Confocal Microscope
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Ideal
for cellular and tissue analysis
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Intrinsic protein fluorescence in the deep-UV (tyrosine,tryptophan)
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Auto-fluorescence of proteins, DNA, and antibodies (GFP,
YFP, CFP, RFP, phycoerythrin)
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Conjugated fluorescence labels and quantum dots
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Fluorescent dyes (rhodamine, fluorescein) and molecular
beacons
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Cell signaling, uptake, and binding
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Nanoparticles (quantum dots and carbon nanotubes)
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Fluorescence Resonance Energy Transfer (FRET)
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Stern-Volmer fluorescence quenching
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Hair, skin, and thin films
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Photosynthesis (porphyrins and chlorophyll)
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Fluorescence
lifetimes and intensity mapping (FLIM)
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TCSPC for ultimate sensitivity
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Wide dynamic range covering lifetimes from < 100 ps to 100 µs
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Incorporates our revolutionary deep-UV pulsed LEDs (280,
295, and 340 nm)
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Plug-and-play components
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Rapid data-acquisition
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Wavelength range from deep-UV to visible (240–850
nm)
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Variable spatial resolution with selectable
pinholes
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Screen-capture shows the fluorescence decay
of Nacetyl-L-tryptophanamide (red). Single-exponential fitting
(green) gives a fluorescence lifetime of 2.9 ns. The excitation
pulse (280 nm LED) is shown in blue.
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Wide wavelength-coverage, from 280–1700 nm
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Standard
UV-visible (340–850 nm) with standard objectives and
detector
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240-850 nm optional with UV objective and optics in
microscope
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Extendable
to near-IR (up to 1700 nm) via fiber-optic coupling to
emission monochromator with near-IR
detectors and near-IR
microscope
optics
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Confocal option with pinhole
turret on emission side and Olympus pinhole accessory on the
excitation side
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x-y-z
scanning with automated stage: resolution 1 µm (< 1 µm
in UV)
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Excitation sources: NanoLEDs from 280–830 nm
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Lifetimes:
TCSPC is 100 ps–100 µs; MCS is > 1 µs
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NanoLED with adapter for bright-field port on the microscope
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TBX detector and C-adapter for the microscope
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