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Protein 295 ex™ - Lifetime Fluorescence Protein Analyzer
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 HORIBA Jobin Yvon, the leader in fluorescence spectroscopy, now
offers the Protein 295 ex™, a lifetime spectrofluorometer that can
measure three fluorescence lifetimes in just a few minutes. The 295
nm LED matches the absorption maxima of the naturally fluorescent
amino acids tryptophan and tyrosine. Thus, the Protein 295 ex™ is
well-suited for examining biomarkers and studying the dynamics of
protein folding. Tryptophan fluores-cence is widely used as a probe
of protein structure and dynamics. Ideal for those biologists and
biochemists who need to test multiple protein samples fast, the Protein
295 ex™ uses Time Correlated Single Photon Counting, a time-resolved
fluorescence technique, to record accurate, reproducible lifetime
data. The Protein 295 ex™ is sensitive enough to detect <100
nM NATA! Analytical software then calculates lifetimes based on least-squares
fitting algorithms. The system uses the modular Fluorocube, with
a pulsed 295 nm UV NanoLED source. NanoLEDs are miniature turn-key
light-sources that generate short optical pulses from <100 ps
to 1 ns.
NanoLED-17: The Protein 295 ex™'s miniature turn-key pulsed
source
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DataStation software makes data-acquisition and analysis on
the Protein 295 ex™ easy. Shown is a fluorescence decay curve,
with a fit giving the fluorescence lifetime.
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Technical Specifications
- NanoLED-17 295 nm LED-based nanosecond pulsed excitation source for
excitation of protein residues tryptophan and tyrosine. A wide
range of interchangeable laser-diode and LED-based excitation sources,
from
UV to near-IR, are available as options.
- Optional faster throughput with FC-OP-Pol2 automatic polarizers
and FC-SC-05 4-cell sample turret
- Motorized excitation optics with large, 50 mm UV-grade fused-silica
lenses, filter-holder for optional 50 mm square filters, and
mount for optional polarizer, for easy op-timization of excitation
beam and high
sensitivity
- Large sample compartment, compatible with cryostats
- Cuvette holder with facility for recirculating-liquid
temperature control
- Optional 4-position cuvette-holder with integrated stirrers,
temperature sensor, and fa-cility for recirculating-liquid
temperature regulation, instead of standard cuvette holder.
Includes four
magnetic stirring beads, power cable, and drive cable.
- Motorized emission optics with 50 mm UV-grade fused-silica
lenses, filter-holder for optional 50 mm square filters,
and mount for optional
polarizer, to optimize emission signal easily
- Low time-dispersion emission monochromator with motorized
wavelength drive, inte-gral safety-shutter interlocked
to lid of sample
compartment to protect the detector, and convenient,
manually adjustable synchronized
slits to record the best signal
- TBX-04 picosecond photon-detection module: spectral
response 185–650 nm; timing walk < 250 ps FWHM; dark-count rate < 80
cps; active area 9 mm diameter. Optional extended-red
(to 850 nm) sensitivity.
- NanoLED pulsed-diode controller module (software
controlled)
- DataStation Hub timing electronics: measures
lifetimes from picoseconds to seconds (depending
on excitation source)
- DataStation data-acquisition software
- DAS6 decay-analysis software: Foundation
Pack (including 1 to 4 exponentials) +
DAS6-8 Anisotropy
- IBM ThinkCenter PC with Windows XP Professional
and 17'' TFT monitor
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